The Museum preserves, protects, studies, and enhances one of the highest expressions of the work of Piero della Francesca. The art and technique of Piero della Francesca are also explained through digital media and videos, and the visitor arrives prepared, at the end of the path, to see the artwork, kept in a protective case.

Furthermore, in the Museum there is the Madonna del latte, a smaller 14th-century fresco, by an unknown local author, depicting a Madonna with Child, discovered in 1911 by the restorer Domenico Fiscali during the detachment of the Madonna del Parto. The museum has an internal shop and a garden, a real balcony overlooking the Monterchi countryside.