The Walker Art Gallery is a Liverpool art gallery, part of the National Museums of Liverpool housing one of the largest collections of paintings in the UK. The Walker Art Gallery was designed by architects Cornelius Sherlock and HH and opened in 1877.

It owes its name to the patron, Sir Andrew Barclay Walker, mayor of the city of Liverpool. The art gallery occupies a neoclassical style building located in William Brown Street, a street entirely dedicated to museums. In the collection there are several works, including Italian paintings by Ercole de 'Roberti, Simone Martini and even Tiziano. Notable works among Italian artists are "Nativity of the Virgin" by Perugino (1472), "Portrait of a man with helmet" by Rosso Fiorentino (1520), "Madonna with Child in Glory" and "Santa Caterina d'Alessandria. "by Vincenzo degli Azani (XVI century)," Adoration of the shepherds "by Mattia Preti (1678) Also not to be missed are the many paintings of the English eighteenth century.

Other international artists include Rembrandt, Poussin, Adam Elsheimer and Degas; The Walker Art Gallery also stands out for having among the richest collections of Pre-Raphaelites in the world.

Among the twentieth-century artists are Lucian Freud, David Hockney and Gilbert and George.

The Walker Art Gallery is also known for organizing important temporary exhibitions.